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    ¿Quiere realizar usted mismo la mezcla de ADN + cebador y enviarnos la placa de 96 pocillos con las muestras para la secuenciación?
    Con este nuevo servicio de secuenciación, usted es quien prepara el ADN y los cebadores en las cantidades correctas. STAB VIDA solamente añadirá el colorante (big-dye) y procederemos a las reacciones cíclicas y  la aplicación del producto PCR en nuestros secuenciadores.


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    • . Esta es una placa propia ya que incorpora un código de barras único.


    • Prepare la mezcla de ADN en cada pocillo (10 ng/100 pb si se trata de un producto de PCR, 100 ng/µl si se trata de un plásmido) y el cebador (10 pmol) y añada agua hasta un volumen total de 15 uL.


    • Haga pedidos online, identifique el código de barras en cada placa y envíenos por correo a la dirección

    PORTUGAL

    Campus FCT UNL Edif. Departamental Química

    Lab 007 Quinta da Torre

    2825-516 CAPARICA

    Si su laboratorio o instituto tiene un punto de recogida, poner sus muestras hasta las 12:00 (en este caso, no habrá gastos de transporte).

    • Enviaremos sus resultados por correo electrónico 24 horas después de recibir la placa en nuestro laboratorio.


    • En este caso el cliente no disfruta de la opción de re-secuenciación de forma gratuita.

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    La calidad de la secuencia está fuertemente influenciada por la calidad de molde de ADN y por el secuenciador utilizado. Nuestro secuenciador de ADN es el ABI 3730 XL, que ofrece la mejor lectura, ya que es, sin duda, el mejor modelo de secuenciador. Además, STAB VIDA utiliza el sistema LongTrace para reducir la tasa de errores y mejorar la longitud de lectura de secuenciación. Nos preocupamos por la calidad de la secuenciación y llegamos frecuentemente a lecturas de alta calidad en longitudes que sobrepasen las 1000 bases.


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    El plazo de entrega de los resultados está principalmente influenciado por la distancia a la que se envíen las muestras. En Portugal y España, el envío tarda aproximadamente 24 horas y los resultados que tarden más de 24 horas serán enviados por correo electrónico. Si usted tiene un punto de entrega diario en su laboratorio o Instituto (enlace), los resultados podrán ser enviados 24 horas después de la entrega de las muestras.

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