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Los oligonucleótidos son enviados en forma seca (liofilizados) y es muy fácil resuspender en solución acuosa. Por tanto, sólo debe disolverse inmediatamente antes de su utilización. Los Oligonucleótidos modificados también se disuelven en agua estéril, pH 7,0 o en uno de los tampones designados.

Tenga en cuenta las siguientes instrucciones:

Es muy importante vortear el contenido antes de abrir el tubo por primera vez. El pellet seco puede perderse si no estuviese en la parte inferior del tubo.
Los oligonucleótidos se conservan mejor en estado seco. Son muy estables, incluso cuando se almacenan a 4 ° C o a temperatura ambiente. Para el almacenamiento de larga duración, los oligonucleótidos se deben almacenar en seco a -20 ° C.
Recomendamos la disolución de los oligonucleótidos en agua estéril a un pH neutro (pH 7,0). Normalmente el agua procedente de los sistemas de purificación de agua tiene un pH ácido, en este caso, se recomienda un tampón, que mantiene un pH constante en un rango ligeramente básico. Recomendamos el uso de TE (10 mM Tris pH 8,0; 0,1 mM EDTA, pH 8,0) * o agua esterilizada para resuspender los oligonucleótidos secos.
El uso de agua con un pH inferior a 7,0 conduce a la depurinización del oligonucleótido. Use NaOH para aumentar el pH o disuelva el oligonucleótido en un tampón apropiado.
Las condiciones de trabajo y un tratamiento adecuados en un ambiente libre de nucleasas garantizan una vida larga para el ARN y el ADN. Esto debe optarse hasta el tratamiento con DEPC. El mayor peligro para los oligonucleótidos es encontrarse con nucleasas. Para minimizar la degradación por nucleasas, deberá almacenar sus oligos a -20 ° C.
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Hay beneficios en la creación de alícuotas de oligonucleótidos en porciones para su uso inmediato y aquellos para su almacenamiento a largo plazo.
Añadiendo TE o agua en diez veces el número de nanomoles dará una concentración final de 100μM. Esta concentración ofrece 100 picomoles de oligonucleótidos porμl. La mayoría de las reacciones de PCR utilizan 10 a 50 picomoles de cada primer por reacción.
El medio más preciso para evaluar la cantidad de oligonucleótidos seguidamente de la síntesis, es medir la densidad óptica del producto final a 260 nm. Este valor se proporciona en la hoja de especificaciones y se determina justo después de la purificación, una vez que los nucleótidos no han sido incorporados ni tampoco los grupos de protección, ya que esto pueden dar lugar a estimaciones imprecisas de la masa de los oligonucleótidos.
Trabajar con concentraciones de 10 pmol / mL o inferiores los hace más inestables. Diluir sólo cantidades mínimas y utilizar solamente una o dos veces.
La frecuente congelación / descongelación debe ser evitada.
Los Oligos marcados con fluorescencia se deben almacenar en la oscuridad.

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