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Com este novo serviço de sequenciação, você é quem prepara o DNA e os primers nas quantidades correctas. A STAB VIDA apenas adicionará o big dye e procederemos às reacções cíclicas, e, à aplicação do produto de PCR nos nossos sequenciadores.

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a placa de 96 poços na STAB VIDA. Esta é uma placa própria uma vez que incorpora um código de barras único.
Prepare a mix de DNA em cada poço (10 ng/100 bp se se trata de um produto PCR; 100/µl ng se se trata de um plasmídeo) e o primer (10 pmol) e adicione água para perfazer um volume total de 15 µL.
Faça a encomenda on-line, identifique o código de barras de cada placa e envie-nos por correio para o endereço:
PORTUGAL
Campus FCT UNL Edif. Departamental Química Lab 007 Quinta da Torre
2825-516 CAPARICA

Se o seu laboratório ou Instituto tem um ponto de recolha, coloque as suas amostras até as 12:00 (neste caso, não haverá custos de transporte).

Enviaremos os seus resultados por e-mail 24 horas após recebermos a placa no nosso laboratório.
Neste caso particular o cliente não usufrui da opção de re-sequenciação grátis.

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A qualidade da sequenciação é fortemente influenciada pela qualidade do template de DNA e pelo sequenciador utilizado. O nosso sequenciador de DNA é o ABI 3730 XL, que proporciona as melhores leituras, uma vez que é sem duvida o melhor modelo de sequenciação. Além disto, a STAB VIDA utiliza o sistema LongTrace para reduzir a taxa de erros e melhorar o comprimento de leitura da sequenciação. Preocupamo-nos pela qualidade da sequenciação e atingimos frequentemente leituras de alta qualidade em comprimentos que superam as 1000 bases.

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O tempo de entrega dos resultados é maioritariamente influenciado pela distância de encomenda das amostras. Em Portugal e Espanha, o transporte demora cerca de 24 hora e os resultados demoram mais 24 horas para serem enviados por e-mail. Se tem um ponto de entrega diário no seu Laboratório ou Instituto (link) os resultados podem ser enviados 24 horas após a entrega das amostras.

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