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cialis viagra levitraA análise de fragmentos, bem como a análise de genotipagem de fragmentos de DNA, permite a identificação de organismos com base em diferenças existentes entre as suas sequencias genómicas. São estudados locus específicos e estes são utilizados para definir o genótipo de cada organismo. Actualmente, as técnicas mais sofisticadas são baseadas na amplificação de regiões polimórficas especificas do genoma por PCR. Este método é bastante comum a vários tipos de aplicações, entre diversas áreas científicas: mapeamento genético de animais, estudos filogenéticos com plantas e microorganismos, medicina forense e diagnóstico.
Na STAB VIDA, oferecemos um serviço de análise de fragmentos (link para backoffice) que inclui a detecção e identificação de Single Nucleotide Polymorphisms (SNP’s), Amplified Fragment Length Polimorfisms (AFLP) e Microsatélites. Os nossos serviços podem incluir uma reacção completa ou uma simples electroforese por capilaridade, e, ao mesmo tempo, damos sugestões e aconselhamento. Teste a flexibilidade da nossa equipa e permita-nos conhecer as especificidades do seu trabalho.


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Short Tandem Repeats (STR), também designados por microsatélites, são regiões do DNA que incluem um número variável consecutivo de sequências repetitivas de 2 a 6 pares de bases. Estas regiões do DNA têm sido escolhidas como marcadores de diagnósticos molecular. A sua aplicação tem vindo a aumentar em outras áreas como na genética de populações, “keen    correlation analysis”, e mapeamento genético. Os microsatélites apresentam algumas características vantajosas: a) são encontrados num número relativamente elevado e b) estão distribuídos regularmente por várias regiões do DNA genómico. Além disso, os STR’s são extremamente heterozigóticos. Na STAB VIDA, a análise dos microsatélites é realizada através da amplificação por PCR de locus específicos utilizando primers marcados com marcadores fluorescentes. Os fragmentos amplificados são depois separados por electrodforese capilar e identificados por emissão fluorescente. Técnicamente, os Microsatélites oferecem diversas vantagens.

 

  1. É possível co-amplificar mais de um locus numa única reacção de PCR.
  2. As pequenas dimensões dos fragmentos permitem uma fácil interpretação de resultados.
  3. A amplificação por PCR torna possível a análise de fragmentos de DNA degradados de quantidades extremamente reduzidas de DNA.
  4. Tempo de análise é curto.

 

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Os SNP’s são pequenas variações nas cadeias de DNA que ocorrem quando há uma alteração num único par de bases do genoma. Estima-se que o genoma humano contém mais de 3 milhões de SNP’s presentes na maioria das pessoas, onde 2/3 destes SNP’s são substituições de uma citosina por uma timina. No entanto, a maioria dos mais importantes grupos de investigação estão a unir esforços no sentido de encontrar e mapear o maior número possível de SNP’s, uma vez que:

  1. Alguns SNP’s flaqueiam mutações que estão associadas com doenças;
  2. De um ponto de vista evolucionário, os SNP’s são extremamente estáveis, pois não sofrem alterações significativas quando são transmitidos à gerações seguintes, o que torna a analise de SNP’s útil no estabelecimento de relações entre grupos populacionais.

Além disto, pensa-se que alguns SNP’s estão associados com a resposta a alguns fármacos. Neste caso, a descoberta e caracterização de SNP’s poderá contribuir para o melhoramento de tratamentos médicos no futuro.


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A STAB VIDA oferece analises high-throughput baseados em ensaios personalizados. A genotipagem pode ser realizada tanto para variações genomicas já conhecidas ou para variações genomicas ainda desconhecidas. Nós armazenamos os ensaios para que os nossos clientes possam transportar facilmente novas series de amostras para analise posterior. As amostras podem ser entregues sob a forma de DNA purificado ou como matéria-prima. Os primers podem ser fornecidos pelo cliente ou desenhados e sintetizados pela STAB VIDA.

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