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NOSSO PROCESSO

Amostras de cliente - Consulta directa com bioquímicos especializados em processamento das amostras, ajudando a definir o protocolo do estudo e a determinar as quantidades apropriadas da amostragem. As amostras são recolhidas pelo cliente e enviadas posteriormente para análise.

Análise Global - As amostras são sujeitas a um processo multi-etapas, incluindo análise usando três plataformas distintas: LC-MS/MS (+ ESI) LC-MS/MS (-ESI) GC-MS.

Identificação - Cada metabolito é automaticamente comparado com um padrão de referência do nosso banco de dados, através do índice de retenção e espectro de massas. O resultado é uma ampla gama de metabolitos identificados, incluindo aminoácidos, carbohidratos, lípidos, ácidos nucléicos e cofactores.

Quantificação -  Após identificação dos metabolitos, o gestor do projeto analisa os dados e avalia a qualidade e, em seguida, aplica análises estatísticas para distinção dos metabolitos com alterações significativas.

Interpretação – O gerente de projeto organiza os metabolitos por vias metabólicas e interpreta as mudanças no contexto da literatura existente e desenho experimental.

RESULTADOS

mVision fornece informação sobre uma ampla gama de metabolitos e a sua variação em resposta a variáveis experimentais.  A informação é entregue ao cliente em várias formas e em diferentes níveis para proporcionar um quadro completo dos efeitos bioquímicos.

PLOTS BIOQUÍMICOS
Para cada amostra, os metabolitos identificados são exibidos em z-plot, proporcionando uma visão de alto nível das alterações nos metabolitos durante a experiência. Um exemplo de z-plot está em baixo demonstrado para um estudo de evolução no tempo, analisando a urina e plasma. Cada ponto representa um diferente metabolito, os pontos vermelhos representam a amostra experimental, e os pontos azuis representam a amostra controlo. O deslocamento ao longo do eixo horizontal representa a extensão da alteração em formato de desvio padrão, a partir da amostra controlo.

ALTERAÇÃO DOS METABOLITOS
O próximo nível de análise envolve a aplicação de análises estatísticas para determinção de mudanças significativas nos metabolitos. Estes “heat maps” permitem uma rápida identificação de metabolitos que aumentam ou diminuem em relação ao controlo. A análise estatística dos metabolitos afectados permite a rápida identificação de áreas para posteriores investigações.

INTERPRETAÇÃO BIOQUÍMICA
Aumentando a complexidade da análise, os metabolitos bioquímicos significativamente alterados são interpretados no contexto de vias metabólicas. Isto permite identificar as vias metabólicas onde estes estão inseridos. O exemplo apresentado demonstra o efeito de uma droga em metabolitos do ciclo do ácido cítrico.

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